(1) Please check if you use the buffer packaged with the device in same kit box.
In the case of using the sample buffer provided from other company, the line will not appear.
In the case of using products of various companies, you have to use the same buffer provided by same kit. If you use other company’s buffer you can not expect the reliable test result.
Because each company has own optimized condition for buffer.
(2) Please check if red color conjugates have been migrated by checking the device window.
If red color conjugates have not been migrated,
it suggests that sample migration has been failed due to less volume of sample. So add more sample for the full migration. If the colorless solution has been migrated,
it suggests that over volume of sample was applied. Try test again by following the insert manual.